Objective: To explore the effects of high-quality nursing intervention on negative emotions and quality of life in gynecological patients after laparoscopy. Methods: A total of 132 gynecological patients after laparoscopy were randomly divided into an observation group (n=66) and a control group (n=66) in a prospective study. The con-trol group received routine nursing care, while the observation group received high-quality nursing intervention. Anxiety, depression, quality of life, postoperative pain, self-care ability, and patient satisfaction were compared between the two groups. Results: The Self-Rating Anxiety Scale and Self-Rating Depression Scale scores were sig-nificantly lower in the observation group compared to the control group (both P<0.001). Pain scores at 6, 24, 48, and 72 hours post-surgery were also lower in the observation group (all P<0.001). The observation group showed significantly higher scores in physical function, general health, social function, emotional role, and mental health (all P<0.001). Furthermore, the observation group demonstrated better self-care skills, self-concept, self-care responsibility, and health knowledge (all P<0.001). Nursing satisfaction during hospitalization was significantly higher in the observation group than in the control group (P<0.05). Conclusion: High-quality nursing intervention is effective in improving depression, anxiety, postoperative pain, and quality of life in gynecological patients after laparoscopy. It also enhances self-care ability and patient satisfaction, making it worthy of clinical promotion and application.